刘林（南开教授）的个人简介
刘林，教授，博士生导师，国家杰出青年基金获得者，南开大学生命科学学院副院长。
刘林教授团队通过完全化学小分子的方法，将卵巢颗粒细胞重编程为具有生殖系转移能力的诱导性多能干细胞，进而分化为卵子，并通过正常受精获得了健康小鼠。该突破属世界首次，为保持生育能力、调节机体内分泌等研究开辟了新思路。
人物经历
1993年北京农业大学（现中国农业大学）获动物生殖学与生物技术博士学位。1994年至1998年分别在英国剑桥Babraham研究所、美国康奈尔大学，康州大学进行博士后和访问学者研究。1998年至2004年，在美国布朗大学妇产科系生殖医学实验室，任研究员、助理教授。1998年至2009年，美国海洋生物学实验室（MBL）兼职研究员。2005年获得国家杰出青年基金资助，2007 年被聘为南开大学生命科学院特聘教授, 兼副院长。
研究方向
在发育生物学和生殖生物技术领域，尤其是在最终能造福人类健康的胚胎工程和再生医学领域，进行基础科学及生物医学应用方面的研究：1）哺乳动物卵子老龄化、早期胚胎发育分子机制与技术工程研究；2）特异多能性干细胞端粒功能及重编程和转基因研究。
科研项目
1、973重大研究计划： 猪诱导多能干细胞（iPS）及其分化发育研究。
2、转基因重大专项： 利用生殖系多能性干细胞构建高效的培育优良品种猪和转基因猪的新技术平台。
3、中加合作交流项目： 卵子及植入前胚胎细胞体积调控与细胞死亡关系的研究。
4、973重大研究计划： 卵巢衰老机理及相关重大疾病的基础研究。
发表论文
代表论文1.Agarwal S, Loh YH, McLoughlin EM, Huang J, Park IH, Miller JD, Huo H, Okuka M, Dos Reis RM, Loewer S, Ng HH, Keefe DL, Goldman FD, Klingelhutz AJ, Liu L, Daley GQ. Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients. Nature 464：292-296（2010）.
2.Huang J, Okuka M, Wang F, Zuo B, Liang P, Kalmbach K, Liu L, Keefe DL. Generation of pluripotent stem cells from eggs of aging mice. Aging Cell Online (2009).
3. C, Chen Z, Liu Z, Huang J, Zhang W, Zhou L, Keefe DL, Liu L. Correlation of expression and methylation of imprinted genes with pluripotency of parthenogenetic embryonic stem cells. Human Molecular Genetics 18:2177-2187 (2009).
4.Liu N, Lu M, Feng XM, Ma FX, Fang ZH, Tian XM, Ren Q, Zhang L, Liu B, Huang PP, Liu L, Han ZC. Exogenous Nanog alleviates but is insufficient to reverse embryonic stem cells differentiation induced by PI3K signaling inhibition. Journal of Cellular Biochemistry 106:1041-1047 (2009).
5.Chen Z, Liu Z, Huang J, Amano T, Li C, Cao S, Wu C, Liu B, Zhou L, Carter MG, Keefe DL, Yang X, Liu L. Birth of Parthenote mice directly from parthenogenetic embryonic stem cells. Stem Cells 27:2136-2145 (2009).
6.Keefe DL, Liu L. Telomeres and reproductive aging. Reproduction, Fertility and Development 21:10-14 (2009).
7.Cao S, Wang F, Chen Z, Liu Z, Mei C, Wu H, Huang J, Li C, Zhou L, Liu L. Isolation and culture of primary bovine embryonic stem cell colonies by a novel method. Journal of Experimental Zoology Part A Ecol Genet Physiol. 311A:368-376 (2009).
8.Chen L, Liu L. Current progress and prospects of induced pluripotent stem cells. Sci China C Life Sci. 52:622-636 (2009).
9.Huang J, Deng K, Wu H, Liu Z, Chen Z, Cao S, Zhou L, Ye X, Keefe DL, Liu L. Efficient production of mice from embryonic stem cells injected into four- or eight-cell embryos by piezo micromanipulation. Stem Cells 26:1883-1890 (2008)
10.Liu L, S. M. Bailey, M. Okuka, P. Mu?oz, C. Li, L. Zhou, C. Wu, E. Czerwiec, L. Sandler, A. Seyfang, M. A. Blasco and Keefe. D. L. Telomere lengthening early in development. Nature Cell Biology 9: 1436-1441 (2007)
11.Liu Y, Wu C, Lyu Q, Yang D, Albertini DF, Keefe DL, Liu L. Germline stem cells and neo-oogenesis in the adult human ovary. Developmental Biology 306:112-120 (2007)
12.Liu L and Keefe DL. Chapter 15 Nuclear Transfer Methods to Study Aging in Methods in Molecular Biology: Biological Aging: Methods and Protocols. Humana Press Inc., Totowa, NJ. Pp191-207 (2007)
13.Keefe DL, Liu L, Marquard K. Telomeres and aging-related meiotic dysfunction in women. Cellular and Molecular Life Sciences 64:139-143 (2007)
14.Wang X, Liu L, Montagna C, Ried T, Deng CX. Haploinsufficiency of Parp1 accelerates Brca1-associated centrosome amplification, telomere shortening, genetic instability, apoptosis, and embryonic lethality. Cell Death & Differentiation 14:924-931 (2007)
15.Dai, Y., L. Wang, H. Wang, Y. Liu, Q. Lyu, N. Li, D.L. Keefe, D.F. Albertini and L. Liu. Abnormalities in centrosome remodeling following somatic cell nuclear transfer (SCNT) in bovine oocytes. Reproduction 131:1051-1061 (2006).
16.Liu, L., Franco, S., Spyropoulos, B., Moens, P.B., Blasco, M.A., and Keefe, D.L. Irregular telomeres impair meiotic synapsis and recombination in mice. Proc Natl Acad Sci U S A 101: 6496-6501 (2004)
17.Liu, L., C.M. DiGirolamo, P.A.A.S. Navarro, M.A. Blasco and D. L. Keefe. Telomerase deficiency impairs differentiation of mesenchymal stem cells. Experimental Cell Research 294: 1-8 (2004)
18.Liu, L., J.R. Trimarchi, P. Navarro, M.A. Blasco and D.L. Keefe. Oxidative stress contributes to arsenic-induced telomere attrition, chromosome instability, and apoptosis. The Journal of Biological Chemistry 278:31998u201332004 (2003)
19.Liu, L., M.A. Blasco, J.R. Trimarchi and D.L. Keefe. An essential role for functional telomeres in mouse germ cells during fertilization and early development. Developmental Biology 249:74-84 (2002)
20.Liu, L., M.A. Blasco and D.L. Keefe. Requirement of functional telomeres for metaphase chromosome alignments and integrity of meiotic spindles. EMBO Reports
21.Liu, L., M. Deng, X.C. Tian, and X. Yang. Chapter 17: Activation of mammalian oocytes: Principles and Practice. In: Introduction to Mammalian Reproduction. Editor: Daulat R.P. Tulsiani, Kluwer academic Publishers, MA, USA. pp-
22.Liu, L., J.R. Trimarchi, P. Smith and D.L. Keefe. Mitochondrial dysfunction leads to telomere attrition and genomic instability. Aging Cell
23.u, L., James R. Trimarchi, Peter Smith and David L. Keefe. Haploidy but not parthenogenetic activation leads to increased incidence of apoptosis in mouse embryos. Biology of Reproduction
24.Trimarchi, J. R., Liu, L., Smith, P.J., and Keefe, D.L. Apoptosis recruits two-pore domain potassium channels used for homeostatic volume regulation. American Journal of Physiology
25.Liu, L., Katherine Hammar, Peter J.S. Smith, Shinya Inoue, David L. Keefe. Mitochondrial modulation of calcium signaling at the initiation of development. Cell Calcium
26.Dinnyés, A., Dai, Y., Barber, M., Liu, L., Xu, J., Zhou, P., and Yang, X. Development of cloned embryos from adult rabbit fibroblasts: effect of activation treatment and donor cell preparation. Biology of Reproduction
27.Liu, L. Cloning efficiency and differentiation. Nature Biotechnology 19:406 (2001) (Correspondence).
28.Liu, L., Oldenbourg, R., Trimarchi, J.R., and Keefe, D.L. A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes. Nature Biotechnology
主要论文陈凌懿,刘林. Current progress and prospects of induced pluripotent stem cells. Science in China
陈凌懿,刘林. 诱导性多潜能干细胞（iPS）的研究现状和展望. 中国科学C辑
Li C, Chen Z, Liu Z, Huang J, Zhang W, Zhou L, Keefe DL, Liu L. Correlation of expression and methylation of imprinted genes with pluripotency of parthenogenetic embryonic stem cells. Human Molecular Genetics
Cao S, Wang F, Chen Z, Liu Z, Mei C, Wu H, Huang J, Li C, Zhou L, Liu L. Isolation and culture of primary bovine embryonic stem cell colonies by a novel method.. Journal of Experimental Zoology Part A Ecol Genet Physiol
Chen Z, Liu Z, Huang J, Amano T, Li C, Cao S, Wu C, Liu B, Zhou L, Carter MG, Keefe DL, Yang X, Liu L*. Birth of Parthenote Mice Directly from Parthenogenetic Embryonic Stem Cells. Stem Cells
Keefe DL, Liu L.. Telomeres and reproductive aging. Reproduction, Fertility and Development
1. Agarwal S, Loh YH, McLoughlin EM, Huang J, Park IH, Miller JD, Huo H, Okuka M, Dos Reis RM, Loewer S, Ng HH, Keefe DL, Goldman FD, Klingelhutz AJ, Liu L, Daley GQ. . Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients. Nature
Huang J, Okuka M, Wang F, Zuo B, Liang P, Kalmbach K, Liu L*, Keefe DL. Generation of pluripotent stem cells from eggs of aging mice.. Aging Cell -
Huang J, Okuka M, McLean M, Keefe DL, Liu L*. . Telomere susceptibility to cigarette smoke-induced oxidative damage and chromosomal instability of mouse embryos in vitro . Free Radical Biology & Medicine
Richards T, Wang F, Liu L, Baltz JM. . Rescue of postcompaction-stage mouse embryo development from hypertonicity by amino acid transporter substrates that may function as organic osmolytes.. Biology of Reproduction
Liu N, Enkemann SA, Liang P, Hersmus R, Zanazzi C, Huang J, Wu C, Chen Z, Looijenga LH, Keefe DL, Liu L.. Genome-wide gene expression profiling reveals aberrant MAPK and Wnt signaling pathways associated with early parthenogenesis.. J Mol Cell Biol
Liu N, Lu M, Feng XM, Ma FX, Fang ZH, Tian XM, Ren Q, Zhang L, Liu B, Huang PP, Liu L, Han ZC.. Exogenous Nanog alleviates but is insufficient to reverse embryonic stem cells differentiation induc. J Cell Biochem
Liu, Z., Hu, Z., Pan, X., Li, M., Togun, T.A., Tuck, D., Pelizzola, M., Huang, J., Ye, X., Yin, Y., Liu, M., Li, C., Chen, Z., Wang, F., Zhou, L., Chen, L., Keefe, D.L., and Liu, L. . Germline competency of parthenogenetic embryonic stem cells from immature oocytes of adult mouse ova. Hum Mol Genet
Huang J, Wang F, Okuka M, Liu N, Ji G, Ye X, Zuo B, Li M, Liang P, Ge WW, Tsibris JC, Keefe DL, Liu L.. Association of telomere length with authentic pluripotency of ES/iPS cells.. Cell Res
Wang F, Yin Y, Ye X, Liu K, Zhu H, Wang L, Chiourea M, Okuka M, Ji G, Dan J, Zuo B, Li M, Zhang Q, Liu N, Chen L, Pan X, Gagos S, Keefe DL, Liu L.. Molecular insights into the heterogeneity of telomere reprogramming in induced pluripotent stem cells. Cell Res.
荣誉记录
科研工作先后获得国家教育部博士点基金，国家自然科学基金，英国海外发展中英技术合作基金，美国农业部，美国卫生部，康乃尔大学及康州大学博士后基金，布朗大学妇女儿童医院教师研究基金，国家杰出青年基金、中国卫生部部属（管）医疗机构临床学科重点、广州市科技计划项目、中国-加拿大国际合作项目、国家重大科学研究计划（973）项目首席科学家、国家重大转基因专项等科研基金的资助。
重要成果
生殖衰老治疗研究获突破
2020-01-05 10:12:17 来源：健康报
